April 16th, 2020 – The “Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids” has been published in STAR Protocols, a Cell press journal.
The protocol, available online, describes the isolation, handling, culture of, and experiments carried on organoids in the context of CF. In CF, the functioning of proteins called cystic fibrosis transmembrane conductance regulator (CFTR) is impaired. The forskolin-induced swelling assay in used to measure the activity of CFTR proteins in organoids, before and after treatment with CFTR modulators. Implementation procedures and validation experiments are described, and representative CFTR genotypes are tested.
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